These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Circ_0001535 Facilitates Tumor Malignant Progression by miR-485-5p/LASP1 Axis in Colorectal Cancer.
    Author: Bai L, Gao Z, Jiang A, Ren S, Wang B.
    Journal: Balkan Med J; 2022 Nov 07; 39(6):411-421. PubMed ID: 36172827.
    Abstract:
    BACKGROUND: Increasing evidence revealed that circular RNAs (circRNAs) are involved in colorectal cancer progression. However, the potential function of circ_0001535 in colorectal cancer remains unclear. AIMS: To investigate the mechanism of circ_0001535 by silencing circ_0001535 in colorectal cancer cells and nude mice. STUDY DESIGN: A cell study. METHODS: Expressions of circ_0001535, LIM and SH3 protein 1 (LASP1) mRNA, and miR-485-5p were detected by real-time quantitative polymerase chain reaction (RT-qPCR). Western blot analyses of LASP1, PCNA, cleaved caspase 3, snail 1, and OCT4 protein expression were performed. CCK-8, EdU, flow cytometry, transwell assays, and sphere formation were conducted to evaluate colorectal cancer cell proliferation, apoptosis, invasion, and stemness. Luciferase reporter assays, RNA pull-down, and RIP validated binding. A nude mice xenograft model was constructed. RESULTS: Circ_0001535 was significantly upregulated in colorectal tissues and cells. Circ_0001535 knockdown suppressed the malignant behavior of colorectal cells such as proliferation, invasion, stemness, and tumor growth in vivo. This knockdown also induced apoptosis by sponging miR-485-5p and upregulating LASP1 expression. CONCLUSION: Circ_0001535 promotes colorectal cancer cell development by absorbing miR-485-5p and upregulating LASP1.
    [Abstract] [Full Text] [Related] [New Search]