These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Emergence of a Hypermucoviscous Klebsiella pneumoniae Strain Coproducing K. pneumoniae Carbapenemase-2 and New Delhi Metallo-β-Lactamase-5 Carbapenemases in Shanghai, China.
    Author: Jiang X, Zhao L, Shen Z, Zhu J.
    Journal: Microb Drug Resist; 2022 Oct; 28(10):980-987. PubMed ID: 36173748.
    Abstract:
    Background: Recently, the emergence of carbapenem-resistant hypermucoviscous Klebsiella pneumoniae has aroused increasing attention in China. We investigated the characteristics of a Klebsiella pneumoniae carbapenemase-2 (KPC-2) and New Delhi metallo-β-lactamase-5 (NDM-5) coproducing hypermucoviscous K. pneumoniae strain, named RJ-8061, which was isolated from the urine of an 86-year-old female patient with pneumonia. Methods: The RJ-8061 strain was investigated by string test, antimicrobial susceptibility testing, polymerase chain reaction for carbapenemase genes detection, capsular genotyping, multilocus sequence typing, whole-genome sequencing, and phylogenetics. A serum killing assay and a Galleria mellonella infection model were used to evaluate the virulence of RJ-8061 in vitro and in vivo. Results: RJ-8061 belonged to the sequence type 11 K64 serotype and showed high-level resistance to almost all frequently used antibiotics, only remaining susceptible to amikacin, colistin, and tigecycline. The complete genome size of RJ-8061 was 6,106,028 bp, including a 5,394,921 bp chromosome and seven circular plasmids. Plasmid pRJ-8061-hybrid is a 294,249 bp hybrid plasmid that co-harbored resistance genes [blaTEM-1B, mph(A), aac(3)-IId] and virulence genes (iucABCDiutA, rmpA2), whereas rmpA2 is a truncated version. In addition, blaKPC-2 and blaNDM-5 were located on plasmids 171,321 bp pRJ-8061-KPC-2 (IncFII/IncR) and 46,161 bp pRJ-8061-NDM-5 (IncX3), respectively. K-mer-based phylogenetic analysis grouped RJ-8061 into a carbapenem-resistant Klebsiella pneumoniae cluster. The G. mellonella infection model revealed that RJ-8061 showed relatively low virulence, with a 50% lethal dose of 106 cfu. Conclusions: To the best of our knowledge, this is the first report of a hypermucoviscous K. pneumoniae coproducing KPC-2 and NDM-5 carbapenemases.
    [Abstract] [Full Text] [Related] [New Search]