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  • Title: Malonate transport in human red blood cells.
    Author: Hajjawi OS, Hider RC.
    Journal: Mol Cell Biochem; 1987 May; 75(1):43-9. PubMed ID: 3627105.
    Abstract:
    Kinetic parameters of [2-14C]malonate uptake by the human erythrocyte membrane have been determined as Km, 24 mM and turnover number, 5 X 10(4) s-1. The translocation of this organic dianion is concentration, pH and temperature dependent. Competitive inhibition of malonate uptake by eosin and inorganic anions, strongly implies that a common route exists for both inorganic anions and organic dianions, namely the anion-exchange Band 3 protein. 14C-Malonate which is nonmetabolized in the erythrocyte, could be a useful probe for monitoring anion-exchange in reconstituted Band 3 systems.
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