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  • Title: Label-Free Aptasensor for Detection of Fipronil Based on Black Phosphorus Nanosheets.
    Author: Huang H, Zhang C, Zhou J, Wei D, Ma T, Guo W, Liu X, Li S, Deng Y.
    Journal: Biosensors (Basel); 2022 Sep 20; 12(10):. PubMed ID: 36290913.
    Abstract:
    A label-free fipronil aptasensor was built based on Polylysine-black phosphorus nanosheets composition (PLL-BPNSs) and Au nanoparticles (AuNPs). A PLL-BP modified glassy carbon electrode (GCE) was fabricated by combining BP NSs and PLL, which included a considerable quantity of -NH2. Au nanoparticles (AuNPs) were placed onto the GCE, and PLL-BPNSs bonded to Au NPs firmly by assembling. The thiolated primers were then added and fixed using an S-Au bond, and competitive binding of the fipronil aptamer was utilized for fipronil quantitative assessment. The sensor's performance was evaluated using differential pulse voltammetry (DPV) method. The linear equation is ΔI (μA) = 13.04 logC + 22.35, while linear correlation coefficient R2 is 0.998, and detection limit is 74 pg/mL (0.17 nM) when the concentration of fipronil is 0.1 ng/mL-10 μg/mL. This aptasensor can apply to quantitative detection of fipronil.
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