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  • Title: A calorimetric investigation of melting of tRNAAsp from brewer's yeast.
    Author: Filimonov VV, Privalov PL, Glangloff J, Dirheimer G.
    Journal: Biochim Biophys Acta; 1978 Nov 21; 521(1):209-16. PubMed ID: 363156.
    Abstract:
    The thermodynamics of tRNAAsp unfolding was studied using a precision scanning microcalorimeter. The overall heat of melting was found to be about 55 J/g irrespective of the ionic strength and magnesium activity. The analysis of complex melting curves obtained in the absence of Mg2+ reveals four successive two-state transitions. The first was identified as the cooperative melting of the tertiary structure and the D region and the others as the melting of individual helical arms.
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