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  • Title: Enzyme-linked immunosorbent assay for detection of antibodies to Rift Valley fever virus in ovine and bovine sera.
    Author: Meegan JM, Yedloutschnig RJ, Peleg BA, Shy J, Peters CJ, Walker JS, Shope RE.
    Journal: Am J Vet Res; 1987 Jul; 48(7):1138-41. PubMed ID: 3631700.
    Abstract:
    An enzyme-linked immunosorbent assay (ELISA) was developed for the rapid detection of antibodies to Rift Valley fever virus (RVFV) in ovine and bovine sera. Conditions to reduce nonspecific reactions were optimized. The ELISA results correlated with those of a plaque-reduction neutralization test, revealing 100% specificity and 90.7% sensitivity. In sera from sheep and cattle inoculated against RVFV, the hemagglutination-inhibition test in combination with the ELISA provided a better indication of response to killed RVFV vaccine than did either test alone.
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