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  • Title: A Simple Method for Inducing Masculinization of Zebrafish Stocks Using 17α-Methyltestosterone.
    Author: Matthews JL, Murphy J, Nasiadka A, Varga ZM.
    Journal: Zebrafish; 2022 Dec; 19(6):241-244. PubMed ID: 36318811.
    Abstract:
    Severely skewed sex ratios in zebrafish stocks can pose significant hurdles for line propagation and sperm cryopreservation. To overcome female-biased sex ratios in stocks derived from imported sperm samples, the Zebrafish International Resource Center has implemented routine supplementation of larval food with 17α-methyltestosterone to skew gonadal sex differentiation toward masculinization. Resulting stocks averaged 80% males.
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