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  • Title: Isolation of murine pluripotent hemopoietic stem cells in the Go phase.
    Author: Miyama-Inaba M, Ogata H, Toki J, Kuma S, Sugiura K, Yasumizu R, Ikehara S.
    Journal: Biochem Biophys Res Commun; 1987 Sep 15; 147(2):687-94. PubMed ID: 3632693.
    Abstract:
    A method to purify pluripotent hemopoietic stem cells in the Go phase from mouse bone marrow was established. Bone marrow cells from 5-fluorouracil (5-FU)-treated mice were fractionated by Percoll density gradient. The cells with density between 1.063 and 1.075 were further separated into wheat germ agglutinin (WGA)-positive and -negative cells using fluorescent-activated cell sorter (FACS) after staining with fluorescein isothiocyanate-conjugated WGA (FITC-WGA). An assay for spleen colony-forming units (CFU-S) revealed that the WGA-positive cells (1 X 10(6)) produced 1380 CFU-S (about 150 times of the number in the original bone marrow cells) on day 12 (but no CFU-S on day 8), whereas the WGA-negative cells produced no CFU-S. Thus, the stem cells in the Go phase are found to be enriched 150 times in 5-FU-treated WGA-positive cells.
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