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  • Title: Generation of Retrogenic Mice to Investigate T Cell Development.
    Author: Gao Y, Bosselut R.
    Journal: Methods Mol Biol; 2023; 2580():199-209. PubMed ID: 36374459.
    Abstract:
    T cells develop in the thymus from bone marrow precursors, and genetic manipulation is an indispensable tool to explore their development in vivo. Retroviral transduction of T cell precursors in the bone marrow can be used to specifically eliminate or enforce gene expression. Here, we describe a fast and efficient method to ectopically express a gene in T cell precursors through retroviral transduction and transplant into recipient mice, which will enable laboratories to evaluate gene function in T cell development in vivo.
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