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  • Title: A rapid objective method for measuring the yeast opsonisation activity of serum.
    Author: Levinsky RJ, Harvey BA, Paleja S.
    Journal: J Immunol Methods; 1978; 24(3-4):251-6. PubMed ID: 363954.
    Abstract:
    A simple, objective semi-quantitative assay for yeast opsonisation by normal polymorphs has been developed. This depends on electronically counting the number of free unphagocytosed yeast particles on a Coulter counter. The method has been compared with the widely used microscope technique and shows excellent correlation. The sera of 112 unselected school-children gave a distribution curve consistent with 2 peaks; 7 gave values less than 2 S.D.s below the mean confirming the high incidence of this immunodeficiency.
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