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  • Title: Aging effect of osteoprotegerin and receptor activator of nuclear factor-κB ligand expression in human periodontal ligament cells under continuous static pressure.
    Author: Wu J, Cui Z, Han Y, Li W.
    Journal: Hua Xi Kou Qiang Yi Xue Za Zhi; 2022 Dec 01; 40(6):654-661. PubMed ID: 36416317.
    Abstract:
    OBJECTIVES: The expression of osteoprotegerin (OPG) and receptor activator of nuclear factor-κB ligand (RANKL) in human periodontal ligament cells (HPDLCs) was investigated by cell culture under continuous static pressure. METHODS: HPDLCs were primarily cultured by tissue explant method and divided into three groups: group A (13-18 years old), group B (19-29 years old), and group C (30-44 years old). CCK-8 was used to detect the proliferation of HPDLCs. The senescence of HPDLCs was detected by senescence-associated β-galactosidase staining. Cells in the three groups were respectively given 0, 1.5, 3, 6, 12, 24, 48, and 72 h of continuous static pressure in vitro. The expression of OPG and RANKL in the supernatant was detected by enzyme-linked immunosorbent assay. RESULTS: After continuous static pressure in vitro for stimulation, the expression of OPG and RANKL changed. The expression of OPG increased with time and age (P<0.01). The expression of RANKL increased with time and decreased with age (P<0.01). The ratio of OPG/RANKL initially decreased, increased with time, and then continued to rise with age (P<0.01). CONCLUSIONS: Aging could increase the expression of OPG and the ratio of OPG/RANKL and decrease the expression of RANKL in HPDLCs under continuous static pressure in vitro. 目的: 通过细胞实验观察持续静压力作用下人牙周膜细胞（HPDLCs）表达骨保护素（OPG）、核因子-κB受体活化因子配体（RANKL）的增龄性变化。方法: 组织块法原代培养HPDLCs，分为A组（13~18岁）、B组（19~29岁）、C组（30~44岁）。CCK-8检测HPDLCs的增殖能力，β-半乳糖苷酶染色检测HPDLCs衰老情况。3组细胞均分别给予0、1.5、3、6、12、24、48、72 h体外持续静压力机械刺激，酶联免疫吸附测定法检测所提上清液中OPG、RANKL的表达。结果: 体外持续静压力刺激后，牙周膜细胞OPG表达水平随加力时间及年龄增长而增加（P<0.01）。RANKL表达水平随加力时间延长而增加，随年龄增长而下降（P<0.01）。OPG/RANKL比值随加力时间呈先下降后增加趋势，且随年龄增长而增加（P<0.01）。结论: 体外持续静压力作用下，增龄因素可上调牙周膜细胞OPG表达及OPG/RANKL比值，下调RANKL表达。. OBJECTIVE: The expression of osteoprotegerin (OPG) and receptor activator of nuclear factor-κB ligand (RANKL) in human periodontal ligament cells (HPDLCs) was investigated by cell culture under continuous static pressure. METHODS: HPDLCs were primarily cultured by tissue explant method and divided into three groups: group A (13-18 years old), group B (19-29 years old), and group C (30-44 years old). CCK-8 was used to detect the proliferation of HPDLCs. The senescence of HPDLCs was detected by senescence-associated β-galactosidase staining. Cells in the three groups were respectively given 0, 1.5, 3, 6, 12, 24, 48, and 72 h of continuous static pressure in vitro. The expression of OPG and RANKL in the supernatant was detected by enzyme-linked immunosorbent assay. RESULTS: After continuous static pressure in vitro for stimulation, the expression of OPG and RANKL changed. The expression of OPG increased with time and age (P<0.01). The expression of RANKL increased with time and decreased with age (P<0.01). The ratio of OPG/RANKL initially decreased, increased with time, and then continued to rise with age (P<0.01). CONCLUSION: Aging could increase the expression of OPG and the ratio of OPG/RANKL and decrease the expression of RANKL in HPDLCs under continuous static pressure in vitro.
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