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  • Title: [Determination of phosphate residues participating in the formation of the spatial structure of tRNA- Leu IAG from cow mammary glands].
    Author: Petrushenko ZM, Tukalo MA, Matsuka GKh.
    Journal: Bioorg Khim; 1986 Nov; 12(11):1492-7. PubMed ID: 3643027.
    Abstract:
    The phosphates of the tRNA-Leu IAG from cow mammary gland (tRNA which has a long variable loop) participating in the formation of three-dimensional structure were studied by alkylation with ethylnitrosourea and methylnitrosourea. A low degree of modification was observed for the phosphates of the following nucleotides: 7, 8, 9, 10 (at the bend site between the acceptor and D-stem); 18, 19, 20A and 21 in the D-loop; 47H and 49 at the joint of variable and T-stem; 57, 58 and 59 in the T-loop.
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