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  • Title: Optimization of ultrasound-assisted extraction of phenolics from Asparagopsis taxiformis with deep eutectic solvent and their characterization by ultra-high-performance liquid chromatography-mass spectrometry.
    Author: Gao H, Wang Y, Guo Z, Liu Y, Wu Q, Xiao J.
    Journal: Front Nutr; 2022; 9():1036436. PubMed ID: 36466400.
    Abstract:
    Asparagopsis taxiformis is a significant source of phenolics. Owing to the incessant demand of green extraction procedures for phenolics from A. taxiformis, ultrasound-assisted extraction (UAE) using deep eutectic solvents (DESs) was optimized. Among the tested DESs, betaine-levulinic acid afforded the highest total phenolic content (TPC). Moreover, the optimal extraction conditions elucidated from single-factor and response surface methodologies comprised a 52.41°C ultrasonic temperature, 46.48% water content of DES, and 26.99 ml/g liquid-to-solid ratio. The corresponding TPC (56.27 mg GAE/100 g DW) and antioxidant ability fitted the predicted values. UAE afforded superior TPC and antioxidant abilities with DESs than with traditional solvents. Using UHPLC-MS, seven phenolic acids, 18 flavonoids, and two bromophenols were identified and quantified. DES-UAE afforded the highest phenolic compound number (26) and sum of contents. These results disclose the high extraction efficiency of DES-UAE for A. taxiformis phenolics and provide a basis for the higher-value application of this species.
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