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  • Title: SERS- and absorbance-based catalytic assay for determination of isocarbophos using aptamer-modified FeMOF nanozyme and in situ generated silver nanoparticles.
    Author: Li C, Yu F, Yang J, Bai H, Ma X, Jiang Z.
    Journal: Mikrochim Acta; 2022 Dec 05; 190(1):4. PubMed ID: 36469128.
    Abstract:
    A new Fe metal-organic framework-loaded liquid crystal 4-octoxybenzoic acid (FeMOF@OCTB) nanosol was synthesized using 1,3,5-phthalic acid, ferrous sulfate, and OCTB as precursors. The FeMOF@OCTB exhibits good stability and strong catalytic effect for the polyethylene glycol 400-Ag (I) indicator reaction, which was evaluated rapidly by the slope procedure. The generated silver nanoparticles have a strong surface-enhanced Raman scattering (SERS) effect and a surface plasmon resonance absorption (Abs) peak at 420 nm. This new bimodal nanosilver indicator reaction was coupled with the isocarbophos (IPS)-aptamer (Apt) reaction. A FeMOF@OCTB nanocatalytic amplified-SERS/Abs bimodal Apt assay for IPS was established. The SERS assay can detect IPS in the concentration range 0.02-1.2 nM, with a detection limit of 0.010 nM. It has been applied to the determination of IPS in rice samples. The relative standard deviation was 4.4-5.8%, and the recovery was 97.7-104%. An Ag nanosol plasmon SERS/Abs dimode aptamer assay was fabricated for trace isocarbophos, based on highly catalysis MOF@OCTB nanoenzyme.
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