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  • Title: Sialyltransferase Activity Assay for Ganglioside GM3 Synthase.
    Author: Inamori KI, Nitta T, Shishido F, Watanabe S, Ohno I, Inokuchi JI.
    Journal: Methods Mol Biol; 2023; 2613():101-110. PubMed ID: 36587074.
    Abstract:
    GM3 synthase (GM3S) is a sialyltransferase that transfers sialic acid from CMP-sialic acid to lactosylceramide. This reaction results in formation of ganglioside GM3 and is essential for biosynthesis of its downstream derivatives, which include a- and b-series gangliosides. Here, we describe a method for GM3S enzymatic assay using fluorescence-labeled alkyl lactoside as acceptor substrate, followed by HPLC for separation of enzymatic product. The method allows quantitative assay of GM3S sialyltransferase activity in cultured cells and mouse brain tissues.
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