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  • Title: Sb-doped FeOCl nanozyme-based biosensor for highly sensitive colorimetric detection of glutathione.
    Author: Chen J, Wu H, Liu J, Su Y, Li H, Lin P, Chen Y, Xiao W, Cao D.
    Journal: Anal Bioanal Chem; 2023 Mar; 415(6):1205-1219. PubMed ID: 36625896.
    Abstract:
    Nanozymes have been emerging as substitutes for natural enzymes to construct biosensors towards biomolecular detection. However, the detection of glutathione (GSH) by nanozyme-based biosensors still remains a great challenge for research on catalytic activity enhancement and the detection mechanism. In this work, Sb-doped iron oxychloride (Sb-FeOCl) with a well-defined nanorod-like structure is prepared by high-temperature calcination. Sb-FeOCl nanorods have high peroxidase-like activity, which can catalyze the decomposition of H2O2 into ·OH and then oxidize 3,3',5,5'-tetramethylbenzidine (TMB). In view of these intriguing observations, a reliable colorimetric method with a simple mixing and detection strategy is developed for the detection of GSH. The linear range of GSH detection is 1-36 μM. The detection limit of GSH reaches a low level of 0.495 μM (3σ/slope). The GSH sensing system also exhibits excellent specificity and anti-interference. Taking advantage of the advantages of the Sb-FeOCl nanorod-based biosensor, it can be used to quantitatively detect GSH levels in human serum. It can be anticipated that the Sb-FeOCl nanorods have broad prospects in the field of enzymatic biochemical reactions.
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