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  • Title: Specificity of non-histone protein antigens in chicken liver nuclei.
    Author: Kiliańska Z, Briggs RC, Hnilica LS.
    Journal: Int J Biochem; 1987; 19(10):995-9. PubMed ID: 3666285.
    Abstract:
    1. Chicken liver nuclei were fractionated by disruption with ultrasound and subsequent precipitation with divatent cations. A small, protein-rich fraction (CS), representing less than 5% of the total nuclear DNA reacted strongly with antisera to dehistonized chicken liver chromatin. 2. Further fractionation of the CS by DNA-affinity chromatography yielded two DNA-binding immunoreactive proteins (approximate Mr 35 and 56 kD). 3. We have shown previously (Kiliańska et al., 1981, Kiliańska, 1984) that this DNA-binding component strongly inhibited the in vitro transcription of RNA.
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