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  • Title: Multi-site phosphorylation of yeast Mif2/CENP-C promotes inner kinetochore assembly.
    Author: Hinshaw SM, Quan Y, Cai J, Zhou AL, Zhou H.
    Journal: Curr Biol; 2023 Feb 27; 33(4):688-696.e6. PubMed ID: 36736323.
    Abstract:
    Kinetochores control eukaryotic chromosome segregation by connecting chromosomal centromeres to spindle microtubules. Duplication of centromeric DNA necessitates kinetochore disassembly and subsequent reassembly on nascent sisters. To search for a regulatory mechanism that controls the earliest steps of this process, we studied Mif2/CENP-C, an essential basal component of the kinetochore. We found that phosphorylation of a central region of Mif2 (Mif2-PEST) enhances inner kinetochore assembly. Eliminating Mif2-PEST phosphorylation sites progressively impairs cellular fitness. The most severe Mif2-PEST mutations are lethal in cells lacking otherwise non-essential inner kinetochore factors. These data show that multi-site phosphorylation of Mif2/CENP-C controls inner kinetochore assembly.
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