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  • Title: [Isolation and properties of the initiation of DNA replication in the sea urchin embryo].
    Author: Brykov VA, Kukhlevskiĭ AD.
    Journal: Mol Biol (Mosk); 1987; 21(5):1346-51. PubMed ID: 3683377.
    Abstract:
    Using the instability of replication loops as a method for the isolation of double-stranded nascent DNA from embryos of the sea urchin Strongylocentrotus intermedius, extruded DNA enriched for replication origins was obtained. The average length of the fragments of the DNA of this fraction was estimated to be about 800 base pairs. The origin-enriched nascent DNA strands were assayed for the presence of inverted repeats. The results show that the origin-enriched DNA is also enriched in inverted repeats. The bulk of palindromes in the total nuclear DNA was estimated to be 200 base pairs in length and from the origin-enriched DNA-150 base pairs.
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