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  • Title: [The small RNP acceptor of glucocorticoids bound to chromatin in liver cell nuclei].
    Author: Konstantinova IM, Volkova IV, Kulichkova VA, Turoverova LV, Gauze LN.
    Journal: Mol Biol (Mosk); 1987; 21(5):1360-6. PubMed ID: 3683379.
    Abstract:
    The buoyant density in the CsCl gradient of the small nuclear RNP tightly bound to chromatin has been studied. It was shown that the buoyant density of alpha-RNP is characteristic for ribonucleoproteins (p = 1.36-1.50 g/cm3). The alpha-particles are of extraordinary stability. These RNP were shown to remain stable under drastic conditions (high ionic strength, SDS, 6 M urea) and resist unfixed caesium chloride density centrifugation. The alpha-RNA hybridizes with total rat liver DNA at C0t1/2 = 10(3). The oligonucleotide analysis of the alpha-RNA shows that the alpha-RNA is heterogeneous.
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