These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Release of enzymes of intermediary metabolism from permeabilized cells: further evidence in support of a structural organization of the cytoplasmic matrix.
    Author: Schliwa M, Euteneuer U, Porter KR.
    Journal: Eur J Cell Biol; 1987 Oct; 44(2):214-8. PubMed ID: 3691549.
    Abstract:
    Cultured BSC-1 cells were exposed to the mild ionic detergent, Brij 58, and the time course of the release of three enzymes of intermediary metabolism (lactate dehydrogenase, aldolase, and creatine phosphokinase) was determined spectrophotometrically. Their release correlates well with the overall decrease in structural complexity of the cytoplasmic matrix. However, each of the three enzymes tested has its own characteristic time-dependent release profile, a result suggesting enzyme-specific variability in their association with the cytomatrix. Cells lysed for 5 min in Brij 58 and then transferred to detergent-free glycolysis medium were able to produce lactate from glucose, an observation consistent with the idea that all enzymes of the glycolytic pathway remained in the cytomatrix. These observations are consistent with a structural rather than viscous organization of the cytoplasm and suggest that cytoplasmic components other than cytoskeletal filaments are able to form parafilamentous, metastable complexes.
    [Abstract] [Full Text] [Related] [New Search]