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  • Title: Evaluation of an in-house IgM/IgG lateral flow assay for serodiagnosis of human brucellosis.
    Author: Shome R, Patra S, Sahib MM, Shanmugam G, Dubey S, Skariah S, Shamshad S, Barman NN, Bora DP, Shome A, Mohandoss N, Shome BR.
    Journal: Indian J Med Microbiol; 2023; 42():55-58. PubMed ID: 36967218.
    Abstract:
    This study aimed to evaluate the diagnostic efficacy of an in-house lateral flow assay (LFA) for the detection of IgM/IgG anti-Brucella antibodies for rapid serodiagnosis of human brucellosis. Three groups of sera samples including 476 from high-risk individuals, 27 from culture-confirmed patients, and 43 from healthy blood donors were used for evaluation of LFA. In comparison with iELISA, the sensitivity, specificity, and accuracy of LFA were >95%, >99%, and 99% respectively. Considering the very good agreement, accuracy, simplicity, and rapidity, LFAs might be useful as a point of care test for the diagnosis of human brucellosis in resource-limited laboratories.
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