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  • Title: The determination of nicotine and cotinine in plasma.
    Author: Davis RA.
    Journal: J Chromatogr Sci; 1986 Apr; 24(4):134-41. PubMed ID: 3700572.
    Abstract:
    A method to determine plasma nicotine and cotinine simultaneously is described. After a simple extraction procedure, the amounts of nicotine and cotinine present in a sample are determined by gas chromatography using nitrogen-sensitive detection and a fused silica capillary column. The method has been demonstrated to detect nicotine and cotinine plasma concentrations within 1 and 9 ng/ml, respectively. The accuracy and precision of the method was demonstrated using spiked calf and human sera. Data are presented for the direct comparison of the present method with other methods of determination for plasma nicotine and cotinine.
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