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  • Title: Production of Water Buffalo SCNT Embryos by Handmade Cloning.
    Author: Palta P, Selokar NL, Chauhan MS.
    Journal: Methods Mol Biol; 2023; 2647():245-258. PubMed ID: 37041339.
    Abstract:
    Cloning by somatic cell nuclear transfer (SCNT) involves the transfer of a somatic nucleus into an enucleated oocyte followed by chemical activation and embryo culture. Further, handmade cloning (HMC) is a simple and efficient SCNT method for large-scale embryo production. HMC does not require micromanipulators for oocyte enucleation and reconstruction since these steps are carried out using a sharp blade controlled by hand under a stereomicroscope. In this chapter, we review the status of HMC in the water buffalo (Bubalus bubalis) and further describe a protocol for the production of buffalo-cloned embryos by HMC and assays to estimate their quality.
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