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  • Title: Binding of lac repressor to the secondary lac operator in Escherichia coli.
    Author: Rambach A, Lebastard M.
    Journal: Mol Gen Genet; 1978 Oct 30; 166(2):229-31. PubMed ID: 370548.
    Abstract:
    In the lac operon, the existence of a secondary repressor binding site, inside Z gene, had been inferred from in vitro binding studies (Reznikoff et al., 1974; Gilbert et al., 1975). A series of deletions have been constructed from a lac transducing lambda bacteriophage. Some of those deleted bacteriophages have still the property of derepressing a chromosomal lac operon, even though they do not contain any more the lac operator. This phenomenon is an indication that the secondary repressor binding site is also active in vivo.
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