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  • Title: Determination of tramadol in human serum by capillary gas chromatography with nitrogen-selective detection.
    Author: Becker R, Lintz W.
    Journal: J Chromatogr; 1986 Apr 25; 377():213-20. PubMed ID: 3711210.
    Abstract:
    A specific, sensitive and precise method for the determination of tramadol in human serum is described. It comprises a three-step extraction procedure and a specific determination by capillary gas chromatography with nitrogen-selective detection, using a homologue of tramadol as an internal standard. The specificity of the method was checked by gas chromatography-mass spectrometry. Precision parameters were 1.7-5.5% (within-run) and 3.2-5.7% (between-run) in the concentration range 12.5-200 ng/ml. The detection limit was about 3 ng/ml.
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