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  • Title: Purification of human milk gangliosides by silica gel chromatography and analysis of trifluoroacetate derivatives by gas chromatography.
    Author: Laegreid A, Kolstø Otnaess AB, Bryn K.
    Journal: J Chromatogr; 1986 Apr 25; 377():59-67. PubMed ID: 3711245.
    Abstract:
    Two of the main gangliosides in human milk were purified by silica gel (230-400 mesh) column chromatography. The gangliosides were identified as GD3 and GM3 by methanolysis (2 M hydrochloric acid; 60 or 85 degrees C) and gas chromatography of trifluoroacetate derivatives on a fused-silica capillary column. The molar ratios of galactose, glucose and sialic acid were 1:1:2 and 1:1:1, respectively, and the sequence in both gangliosides comprised sialic acid--galactose--glucose--ceramide, as indicated by the time course of cleavage of individual components during methanolysis at 60 degrees C.
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