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  • Title: Superresolution Imaging with Single-Antibody Labeling.
    Author: Gunasekara H, Perera T, Anderson J, Saed B, Ramseier N, Keshta N, Hu YS.
    Journal: Bioconjug Chem; 2023 May 17; 34(5):825-833. PubMed ID: 37145839.
    Abstract:
    We present a versatile single-molecule localization microscopy technique utilizing time-lapse imaging of single-antibody labeling. By performing single-molecule imaging in the subminute time scale and tuning the antibody concentration to create sparse single-molecule binding, we captured antibody labeling of subcellular targets to generate superresolution images. Single-antibody labeling enabled dual-target superresolution imaging using dye-conjugated monoclonal and polyclonal antibodies. We further demonstrate a dual-color strategy to increase the sample labeling density. Single-antibody labeling paves a new way to evaluate antibody binding for superresolution imaging in the native cellular environment.
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