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  • Title: Quantifying CBM-Carbohydrate Interactions Using Microscale Thermophoresis.
    Author: Wu H, Montanier CY, Dumon C.
    Journal: Methods Mol Biol; 2023; 2657():103-114. PubMed ID: 37149525.
    Abstract:
    Microscale thermophoresis (MST) is an emerging technology for studying a broad range of biomolecular interactions with a high sensitivity. The affinity constant can be obtained for a wide range of molecules within minutes based on reactions in microliters. Here we describe the application of MST in quantifying protein-carbohydrate interactions. A CBM3a and a CBM4 are titrated with insoluble substrate (cellulose nanocrystal) and soluble oligosaccharide (xylohexaose), respectively.
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