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  • Title: Isolation of a thiamine-binding protein from Saccharomyces cerevisiae.
    Author: Iwashima A, Nishimura H.
    Journal: Biochim Biophys Acta; 1979 Mar 27; 577(1):217-20. PubMed ID: 371687.
    Abstract:
    Thiamine-binding protein was isolated from Saccharomyces cerevisiae by successive procedures of cold osmotic shock treatment, DEAE-cellulose chromatography and ultrafiltration. The purified thiamine-binding protein was an electrophoretically homogeneous molecule which appeared to be a glycoprotein with a molecular weight of 140 000 by sodium dodecyl sulfate polyacrylamide gel electrophoresis. No thiamine-binding protein was observed by disc gel electrophoresis in the shock fluid released from yeast cells grown in the presence of 1 muM thiamine, indicating that the formation of this protein is regulated by exogenous thiamine as previously suggested.
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