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  • Title: Detecting Mycoplasma bovis in milk by enzyme-linked immunosorbent assay, using monoclonal antibodies.
    Author: Boothby JT, Mueller R, Jasper DE, Thomas CB.
    Journal: Am J Vet Res; 1986 May; 47(5):1082-4. PubMed ID: 3717730.
    Abstract:
    Enzyme-linked immunosorbent assay, using monoclonal antibodies, was used to detect Mycoplasma bovis in milk samples from a dairy experiencing an epizootic of mastitis. This method was specific (100%) for M bovis. Broth enrichment increased the sensitivity from 65% to 86%, compared with standard culture methods.
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