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  • Title: A comparative study of buffer systems as cryoprotectants for human spermatozoa.
    Author: Prins GS, Weidel L.
    Journal: Fertil Steril; 1986 Jul; 46(1):147-9. PubMed ID: 3720973.
    Abstract:
    Several cryoprotective media currently in use for human sperm cryopreservation have been directly compared with each other by use of a static vapor freezing technique. The TEST-citrate-yolk buffers with glycerol resulted in the highest motility and longevity after thawing; thus, their use is recommended.
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