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  • Title: PMN migration measured by bottom filter counts in modified Boyden chambers. Chemokinetic deactivation and vinblastine inhibition of chemotaxis.
    Author: Bandman U, Norberg B, Eriksson S.
    Journal: Haematologia (Budap); 1986; 19(1):13-9. PubMed ID: 3721320.
    Abstract:
    Polymorphonuclear leukocyte (PMN) migration and chemotaxis were studied by a modified Boyden chamber technique in 100% autologous serum with casein as attractant. PMN chemotaxis was induced by increasing the top gradient concentration of casein to 20 mg/ml. Under non-gradient condition, casein 20 mg/ml inhibited PMN migration, which was thought to be due to chemotactic deactivation. Vinblastine 0.01 microgram/ml partially inhibited PMN migration in a casein gradient with a top gradient concentration of 20 mg casein/ml. It is suggested that PMN migration in the casein gradient was composed of both vinblastine-sensitive chemotaxis and vinblastine-resistant chemotaxis.
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