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  • Title: Different substrate specificities of the two DNA ligases of mammalian cells.
    Author: Arrand JE, Willis AE, Goldsmith I, Lindahl T.
    Journal: J Biol Chem; 1986 Jul 15; 261(20):9079-82. PubMed ID: 3722188.
    Abstract:
    Mammalian cells contain the DNA ligases I and II. These enzymes show different molecular weights and heat labilities, and antibodies against ligase I do not inhibit ligase II. Here, the nonidentical substrate specificities of the enzymes are described. Under standard reaction conditions DNA ligase I, but not ligase II, catalyzes blunt-end joining of DNA, while ligase II is the only activity that joins oligo(dT) molecules hydrogen-bonded to poly(rA). These differences facilitate the distinction between the two enzymes and should permit further analysis of their functions.
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