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  • Title: Ratiometric optical detection of pyrophosphate based on aggregation-caused dual-signal response of gold nanoclusters.
    Author: Cao HT, Zhao T, Liu MQ, Guo LZ, He YQ, Zhang K, Mai X, Li N.
    Journal: Luminescence; 2023 Aug; 38(8):1458-1464. PubMed ID: 37222209.
    Abstract:
    Sensing of pyrophosphate ion (PPi) has received much attention due to the strong demand for clinical diagnostics. Here, based on gold nanoclusters (Au NCs), a ratiometric optical detection method for PPi is developed by simultaneously detecting the dual signals of fluorescence (FL) and second-order scattering (SOS). The PPi is detected by inhibiting the formation of aggregates of Fe3+ with Au NCs. Binding of Fe3+ to Au NCs causes aggregation of Au NCs, which leads to fluorescence quenching and scattering increasing. The presence of PPi can competitively bind Fe3+ to re-disperse the Au NCs and finally recover the fluorescence and reduce the scattering signal. The designed PPi sensor shows a high sensitivity with a linear range 5-50 μM and a detection limit of 1.2 μM. In addition, the assay has excellent selectivity for PPi, which makes its application in real biological samples extremely valuable.
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