These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Transplantation of bovine mammary tissue to athymic nude mice: growth subcutaneously and in mammary gland-free fat pads.
    Author: Sheffield LG, Welsch CW.
    Journal: J Dairy Sci; 1986 Apr; 69(4):1141-7. PubMed ID: 3722534.
    Abstract:
    Mammary epithelium from five Holstein cows was transplanted as subcutaneous slices and as collagenase dissociated epithelial cells into mammary gland free-fat pads of athymic nude mice. Two weeks posttransplantation, mice were injected daily for 20 d with saline, 17 beta-estradiol plus progesterone, or 17 beta-estradiol plus progesterone plus growth hormone plus prolactin. In a second experiment, mice were treated with saline, cholera toxin, 17 beta-estradiol (subcutaneous pellet of 2 mg 17 beta-estradiol and 38 mg cholesterol), or 17 beta-estradiol plus cholera toxin. In each experiment mammary slices maintained normal morphology. Growth of epithelium within slices ([3H]thymidine autoradiography) was increased 167% by estradiol plus progesterone, 264% by estradiol plus progesterone plus growth hormone plus prolactin, 90% by estradiol, 60% by cholera toxin, and 137% by estradiol plus cholera toxin. Cells injected into mammary gland free fat pads formed hollow, multilayered, spherical "organoids." Organoid area was increased 47% by estradiol plus progesterone, 189% by estradiol plus progesterone plus growth hormone plus prolactin, 72% by estradiol, 86% by cholera toxin and 74% by estradiol plus cholera toxin. Thus, athymic nude mice appear suitable for the ex vivo in vivo study of bovine mammary epithelial growth and differentiation.
    [Abstract] [Full Text] [Related] [New Search]