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  • Title: Determination of benzoylecgonine in urine from drug abusers using ion pair high performance liquid chromatography.
    Author: Svensson JO.
    Journal: J Anal Toxicol; 1986; 10(3):122-4. PubMed ID: 3724072.
    Abstract:
    An ion pair reversed-phase high performance liquid chromatographic (HPLC) method for the determination of the cocaine metabolite benzoylecgonine in urine is presented. It includes sample purification with solid phase extraction (Sep-Pak C18) and ultraviolet (UV) detection at 233 nm. It is a relatively simple and sensitive method developed for confirmation of positive results from radioimmunoassay screening for benzoylecgonine in urine from drug abusers. Detection of benzoylecgonine concentrations down to 20 ng/mL is possible. Linear standard curves have been obtained from 30 ng/mL to 6 micrograms/mL.
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