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  • Title: Rapid diagnosis of tick-borne encephalitis by means of enzyme linked immunosorbent assay.
    Author: Hofmann H, Frisch-Niggemeyer W, Heinz F.
    Journal: J Gen Virol; 1979 Mar; 42(3):505-11. PubMed ID: 372494.
    Abstract:
    An enzyme-linked immunosorbent assay was applied for determining separately IgM and IgG antibodies against tick-borne encephalitis virus. A micro-modification in microtitre plates proved to be at least as sensitive as the HI test. However, more precise information could be achieved by a macrotest using antigen coated polystyrene balls. False positive results in IgM antibody determinations could be caused by a rheumatoid factor. A high content of IgM antibodies in a serum could impair the determination of its IgG antibodies but not vice versa. Titres were expressed in comparison to a positive control serum.
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