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  • Title: Leishmania in the Old World: 2. Heterogeneity among L. tropica zymodemes.
    Author: Le Blancq SM, Peters W.
    Journal: Trans R Soc Trop Med Hyg; 1986; 80(1):113-9. PubMed ID: 3726972.
    Abstract:
    Isoenzyme profiles of 27 stocks of Leishmania tropica from widely separated geographical areas were compared with those of reference strains of L. tropica and L. major using starch-gel electrophoresis of 13 enzymes (GPI, GD, ES, PGM, PEPD, NH, ASAT, ALAT, PK, MPI, 6PGD, SOD, MDH). 18 zymodemes were seen. L. tropica showed considerable intraspecific variation which did not correlate with its epidemiological uniformity. Isolates from cases of cutaneous and visceral leishmaniasis and leishmaniasis recidivans were identified as L. tropica. Only one isoenzyme band was held in common with the enzyme profile of the L. major reference strain thus supporting the status of L. tropica as a separate species.
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