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  • Title: [Effect of cryopreservation on the acrosomal enzyme activity of the spermatozoa of rams].
    Author: Tsekova E, Spasov Kh, Angelova M, Georgiev GKh.
    Journal: Vet Med Nauki; 1986; 23(1):73-6. PubMed ID: 3727374.
    Abstract:
    Studied was the effect of cryoconservation on the activity of acrosine and acrosomal acid phosphatase of spermatozoa of rams stored in the form of pellets with the use of a diluent after Nagaze-Niva. It was found that the activity of acid phosphatase dropped by 35 per cent when stored in the course of 24 hours and 7 days. Acrosine was shown to be more stable at cryoconservation--its activity did not change under the same conditions of storing, however, it dropped by 30 per cent for 7 days. Admittedly, one of the reasons for the drop of the fertilizing capacity of ram spermatozoa after freezing was the drop of acrosomal enzyme activity. Most probably, the noxious factors were two--ultralow temperatures, and storage duration of spermatozoa.
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