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  • Title: Occurrence of Lectin in the Silkgland of the Silkworm, Bombyx mori: (lectin/silkgland/hemolymph).
    Author: Amanai K, Sakai M, Sakurai S, Mori T, Nikaido O, Ohtaki T.
    Journal: Dev Growth Differ; 1991 Aug; 33(4):421-427. PubMed ID: 37280979.
    Abstract:
    Monoclonal antibodies were prepared against the 350 kDa lectin purified from larval hemolymph of the silkworm, Bombyx mori. The antibodies inhibited the hemagglutinating activity (HA activity) and bound specifically to the hemolymph 350 kDa lectin on Western blotting analysis. Immunohistological observations revealed the occurrence of lectin in the cuticular intima of the anterior silk gland, but not the middle or posterior silk glands of fifth instar larvae of Bombyx mori. Extracts from the anterior silk glands showed HA activity and exhibited the same biochemical characteristics as those of the 350 kDa lectin in the hemolymph. These results suggested that lectin-like molecules in epithelial tissues may be important in histolysis during molting and metamorphosis.
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