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  • Title: [Study of human erythrocyte membrane proteins by 2-dimensional electrophoresis].
    Author: Gromov PS, Shandala AM, Kovalev LI, Shishkin SS.
    Journal: Biull Eksp Biol Med; 1986 Jul; 102(7):28-30. PubMed ID: 3730587.
    Abstract:
    Fractionation of human erythrocyte membrane proteins was performed using a modification of two-dimensional gel electrophoresis described by P. O'Farrel with isoelectric point plotted against molecular mass. All major erythrocyte proteins, including high molecular weight proteins, such as spectrin and band 3 protein, identified by one-dimensional sodium dodecyl sulfate gel electrophoresis, were visualized by silver staining of two-dimensional gels. All in all about 50 polypeptides were distinguished on two-dimensional electrophoretic patterns. Preliminary protein map was developed.
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