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  • Title: Synergistic effect of ammonium and potassium ions on pyruvate kinase from Ehrlich ascites tumor cells.
    Author: Olavarría JS, Artacho E, Sánchez F, Martínez P, Núñez de Castro I.
    Journal: Enzyme; 1986; 35(1):34-41. PubMed ID: 3732223.
    Abstract:
    The effect of NH4+ on M2-pyruvate kinase isolated from Ehrlich ascites tumor cells was investigated. The enzyme is activated by NH4+ more efficiently than by K+. Moreover, a synergistic interaction of the two cations is observed since NH4+ increases the affinity of the enzyme for K+. The affinity of the enzyme for phosphoenolpyruvate is also increased in the presence of NH4+, and in these conditions the activating effect of fructose-1,6-bisphosphate is reduced. It is proposed that NH4+ be considered a specific allosteric activator of the tumor enzyme.
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