These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Rational development of a peptide-based probe for fluorescence and colorimetric dual-mode detection of Cu2+ and S2- ions: Real application in cell imaging and test strips.
    Author: Zheng M, Zhou M, Xue S, Chen B, Wang P.
    Journal: Spectrochim Acta A Mol Biomol Spectrosc; 2023 Dec 05; 302():123006. PubMed ID: 37369144.
    Abstract:
    A new dual-mode probe FAM-SSH with fluorescence and colorimetric properties was developed by solid-phase peptide synthesis, comprising 5-carboxy fluorescein (5-FAM) as a fluorophore, and tripeptide (Ser-Ser-His) as a recognition group. FAM-SSH not only displayed highly selective detection of Cu2+ based on fluorescence quenching mode, but also achieved colorimetric recognition of Cu2+ in solution, wherein a color change was observable to the naked eye. Additionally, the FAM-SSH-Cu2+ ensemble was highly selective for S2- over a wide pH range (7.0-12.0), characterized by a fluorescence enhanced response and colorimetric recognition, which was caused by the release of FAM-SSH and the precipitation of CuS. Moreover, the limit of detection (LOD) values for Cu2+ and S2- were 55.5 nM and 31.1 nM, respectively. Results of sample analyses and cell imaging experiments indicated that FAM-SSH has exciting field practicability and good cell permeability, and would be further useful for detection and imaging in environmental systems and living cells. Finally, test strips were produced by immersing them in FAM-SSH solution, thereby creating a method for portable visual detection. More importantly, a smartphone-assisted visual sensing platform was also developed for semi-quantitative Cu2+ and S2- detection with LOD values of 0.48 μM and 1.22 μM, respectively.
    [Abstract] [Full Text] [Related] [New Search]