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  • Title: T4PPVB-COP composite-driven innovative electrochemiluminescence aptasensor for ultra-sensitive detection of chlorpyrifos.
    Author: Li Y, Hu Q, Zhang J, Zhou H, Wang N, Fang Y, Cui B.
    Journal: Food Chem; 2023 Nov 30; 427():136713. PubMed ID: 37390738.
    Abstract:
    Herein, an enhanced electrochemiluminescence (ECL) aptasensor driven by a complex (T4PPVB-COP@CdS QDs) with large specific surface area and high stability was constructed for highly sensitive detection of chlorpyrifos (CPF), using electrostatic interactions and signal amplification techniques. In the presence of CPF, the specific binding between the aptamer and CPF caused partial detachment of the aptamer from the sensor, thus restoring the ECL signal. Notably, gold nanoparticles functionalized with streptavidin (SA) as signal enhancers further amplified the ECL signal in specific interactions with aptamers, thereby improving the sensitivity of the assay. Based on this, the proposed ECL aptasensor demonstrated significant detection performance for CPF with a linear range of 1-107 pg/mL and a LOD of 0.34 pg/mL. Furthermore, the feasibility of the ECL aptasensor was validated by the detection and analysis of CPF in real samples, which also provided a broad reference value for bioanalysis.
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