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  • Title: The phage SPO1-specific RNA polymerase, E.gp28, recognizes its cognate promoters in thymine-containing DNA.
    Author: Romeo JM, Greene JR, Richards SH, Geiduschek EP.
    Journal: Virology; 1986 Aug; 153(1):46-52. PubMed ID: 3739226.
    Abstract:
    The bacteriophage SPO1 gene 28-encoded protein, gp28, directs specific recognition of viral middle promoters in hydroxymethyluracil-containing DNA by the Bacillus subtilis host's RNA polymerase core. Using appropriately sensitive methods of detection, we have shown that discrimination against thymine-containing DNA is not absolute and that the gp28-containing RNA polymerase precisely initiates transcription at two thymine-containing SPO1 middle promoters.
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