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  • Title: Mechanism of enhanced enzymatic hydrolysis performance by ethanol assisted deep eutectic solvent pretreatment- from the perspective of lignin.
    Author: Yao L, Chai M, Cui P, Geun Yoo C, Yuan J, Meng X, Yang H.
    Journal: Bioresour Technol; 2023 Oct; 385():129461. PubMed ID: 37423545.
    Abstract:
    Valorization of lignocellulose has received a lot of attention due to the abundance of lignocellulosics. It was showed that synergistic carbohydrate conversion and delignification could be achieved via ethanol assisted DES (choline chloride/lactic acid) pretreatment. To explore the reaction mechanism of lignin in the DES, milled wood lignin obtained from Broussonetia papyrifera was subjected to pretreatment at critical temperatures. The results suggested that ethanol assistance could contribute the incorporation of ethyl groups and reduce condensation structures of Hibbert's ketone. Adding ethanol at 150 °C not only decreased formation of condensed G unit (from 7.23% to 0.87%), but also removed J and S' substructures, thus effectively reducing the adsorption of lignin on cellulase, and promoting the glucose yield after enzymatic hydrolysis.
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