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  • Title: CuSO4-TiO2 as Kjeldahl digestion catalyst in manual determination of crude protein in animal feeds.
    Author: Kane PF.
    Journal: J Assoc Off Anal Chem; 1986; 69(4):664-6. PubMed ID: 3745097.
    Abstract:
    The official AOAC manual Kjeldahl methods for determining crude protein in animal feeds have several disadvantages. For the HgO catalyst method, there are environmental concerns and a lengthy digestion. For the CuSO4 catalyst method, the digestion period is shorter, but still 90 min. A different catalyst combination, CuSO4-TiO2, makes 40 min digestion feasible. Comparison of these catalysts on a group of representative feeds resulted in a mean difference, Cu-Ti minus HgO, of 0.034% protein. Standard deviation of the differences was 0.36. A Student's t-test showed no significant difference. The method will be collaboratively studied.
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