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  • Title: Influence of chloramine T iodination on the biological and immunological activity or the molecular radius of the human growth hormone molecule.
    Author: Bartolini P, Ribela MT.
    Journal: J Immunoassay; 1986; 7(3):129-38. PubMed ID: 3745443.
    Abstract:
    Potential alterations of the somatotropic activity of human growth hormone (hGH) resulting from Chloramine T labelling reaction, iodination up to 2.7 atoms/molecule and indirect radiation effects, have been studied. Three 2X2 factorial assays, performed in hypophysectomized rats, failed to reveal any significant difference (P greater than 0.05) in true growth promoting activity between hGH and (127-I)hGH, even after storing the latter with 125-I. Similar results were obtained applying a sensitive and precise gel filtration technique for Stokes Radius determination and radioimmunoassay.
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