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  • Title: Within-day variation of lipolytic activity in human milk.
    Author: Lavine ME, Clark RM, Hundrieser KE, Ferris AM.
    Journal: J Dairy Sci; 1986 Jul; 69(7):1784-6. PubMed ID: 3745580.
    Abstract:
    Milk was collected from six mothers during their 8th wk of lactation to determine the diurnal variation of bile salt-stimulated lipase and serum-stimulated lipase. On the day of collection, one breast was completely emptied at 0600, 1000, 1400, 1800, and 2000 h. Activities of bile-stimulated lipase and serum-stimulated lipase in the milk were determined. Activity of bile-stimulated lipase was constant throughout the day, whereas serum-stimulated lipase activity varied significantly with time. Both activities differed significantly in milk from different women. Time of collection must be considered when measuring activity of serum-stimulated lipase but is not a major variable when measuring activity of bile-stimulated lipase in human milk. These results may be helpful when designing future studies involving these enzymes.
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